225 research outputs found
A Violência no Local de Trabalho em Instituições de Saúde: Um Estudo Monocêntrico sobre Causas, Consequências e Estratégias de Prevenção
Introduction: Workplace violence is one of the main risk factors in the professional world. Healthcare workers are at higher risk when
compared to other sectors. Our study aimed to characterize physical and verbal violence in a public hospital and to define occupational
health prevention and surveillance strategies.
Material and Methods: Single center observational cross-sectional study, carried amongst healthcare workers in a public hospital in
Lisbon. A qualitative survey was carried out through six in-depth interviews. A quantitative survey was carried through questionnaires
delivered to 32 workers. A significance level of 5% was accepted in the assessment of statistical differences. The Mann-Whitney test
and the Fisher’s exact test were used to calculate p values.
Results: The main results are: (1) 41 violence incidents were reported in the quantitative phase; (2) 5/21 [23.81%] victims notified
the incident to the occupational health department; (3) 18/21 [85.71%] victims reported a permanent state of hypervigilance; (4) 22/28
[78.57%] participants self-reported poor or no familiarity with internal reporting procedures; (5) 24/28 [85.71%] participants believed it
is possible to minimize workplace violence.
Discussion: Workplace violence is favored by unrestricted access to working areas, absence of security guards and police officers
or scarce intervention. The low notification rate contributes to organizational lack of action. The state of hypervigilance reported in our
study reflects the negative effects of threatening occupational stressors on mental health.
Conclusion: Our results show that workplace violence is a relevant risk factor that significantly impacts workers’ health in a noxious
manner, deserving a tailored occupational health approach whose priority areas and strategies have been determined.Introdução: A violência no local de trabalho é um dos principais fatores de risco no mundo do trabalho. Os trabalhadores da saúde
apresentam um risco superior. O nosso estudo teve como objetivo caracterizar a violência fÃsica e verbal num hospital público e definir
estratégias de prevenção e vigilância em saúde ocupacional.
Material e Métodos: Estudo observacional transversal monocêntrico, conduzido num hospital público em Lisboa com trabalhadores
da saúde. Foi realizado um inquérito qualitativo com entrevistas em profundidade a seis trabalhadores e um inquérito quantitativo
com questionários a 32 trabalhadores. Aceitou-se um nÃvel de significância de 5% na avaliação das diferenças estatÃsticas. O teste de
Mann-Whitney e o teste exato de Fisher foram usados para calcular os valores de p.
Resultados: Os principais resultados são: (1) 41 episódios reportados na fase quantitativa; (2) 5/21 [23,81%] vÃtimas notificaram o incidente; (3) 18/21 [85.71%] vÃtimas reportaram estados de hipervigilância permanente; (4) 22/28 [78,57%] participantes não conheciam
ou conheciam mal os procedimentos de notificação; (5) 24/28 [85,71%] consideravam possÃvel minimizar o problema.
Discussão: A violência é favorecida pelo acesso livre à s zonas de trabalho, ausência de agentes de segurança e polÃcia ou falta da
respetiva intervenção. A baixa notificação contribui para a ausência de medidas organizacionais. O estado de hipervigilância relatado
reflete o efeito prejudicial da exposição a fontes de stress e ameaça.
Conclusão: A violência no local de trabalho é um fator de risco relevante, com impacto negativo na saúde dos trabalhadores e merece
uma abordagem individualizada no âmbito da saúde ocupacional, cujas áreas e estratégias prioritárias foram definidas neste estudo.
Palavras-chave: Fatores de Risco Profissionais; Prevenção; Saúde Ocupacional; Trabalhadores da Saúde; Violência no Local de trabalho.info:eu-repo/semantics/publishedVersio
Sediment-matrix igneous breccias at the top contacts of felsic units in the IPB : implications for VHMS exploration
The Volcanic Sedimentary Complex of the Iberian Pyrite Belt is dominated by mudstone units and comprises felsic lavas/domes and pyroclastic units that define lava-cryptodome-pumice cone volcanoes. Sediment-matrix igneous breccias may outline the contacts of volcanic units, occur within them, or lie laterally to the volcanic centres. These breccias can form by several processes, each with its genetic implications, having nevertheless very similar final aspect. We have distinguished and characterized several sediment-matrix breccia types. The most abundant types are sediment-infill volcanic breccia and peperite; however other types of sediment-matrix breccia were also identified. The correct identification of these breccias is crucial to reconstruct the volcanic centres and to define the stratigraphy, which in mineralized volcanic provinces is a major issue both for metallogenic and mineral exploration models
Protein glycosylation and tumor microenvironment alterations driving cancer hallmarks
Decades of research have disclosed a plethora of alterations in protein glycosylation that decisively impact in all stages of disease and ultimately contribute to more aggressive cell phenotypes. The biosynthesis of cancer-associated glycans and its reflection in the glycoproteome is driven by microenvironmental cues and these events act synergistically toward disease evolution. Such intricate crosstalk provides the molecular foundations for the activation of relevant oncogenic pathways and leads to functional alterations driving invasion and disease dissemination. However, it also provides an important source of relevant glyco(neo)epitopes holding tremendous potential for clinical intervention. Therefore, we highlight the transversal nature of glycans throughout the currently accepted cancer hallmarks, with emphasis on the crosstalk between glycans and the tumor microenvironment stromal components. Focus is also set on the pressing need to include glycans and glycoconjugates in comprehensive panomics models envisaging molecular-based precision medicine capable of improving patient care. We foresee that this may provide the necessary rationale for more comprehensive studies and molecular-based intervention.The authors wish to acknowledge the Portuguese Foundation for Science and Technology (FCT) for the human resources grants: PhD grant SFRH/BD/111242/2015 (AP), and FCT auxiliary researcher grant CEECIND/03186/2017 (JF). FCT is co-financed by European Social Fund (ESF) under Human Potential Operation Programme (POPH) from National Strategic Reference Framework (NSRF). The authors also acknowledge the Portuguese Oncology Institute of Porto Research Centre (CI-IPOP-29-2014; CI-IPOP-58-2015), the PhD Program in Biomedical Sciences of ICBAS-University of Porto, and the Early stage cancer treatment, driven by context of molecular imaging (ESTIMA) framework (NORTE-01-0145-FEDER-000027). The authors were also supported by the CANCER project (NORTE-01-0145-FEDER-000029) co-funded through the NORTE-45-2015-02
A Protocol for FRET-Based Live-Cell Imaging in Microglia
This protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling events to be studied in real time with exquisite subcellular resolution. However, FRET-based live-cell imaging requires application-specific biosensors and specialized imaging systems, limiting its use in in vivo studies. For complete details on the use and execution of this protocol, please refer to Socodato et al. (2020), Portugal et al. (2017), and Socodato et al. (2018).This work was financed by FEDER (Fundo Europeu de Desenvolvimento Regional) funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia)/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project POCI-01-0145-FEDER-031318 (PTDC/MED-NEU/31318/2017). The authors acknowledge the support of the following: i3S Scientific Platform: Advanced Light Microscopy (ALM), members of the national infrastructure PPBI-Portuguese Platform of BioImaging (supported by POCI-01–0145-FEDER-022122). C.C.P. and R.S. hold employment contracts financed by national funds through FCT – Fundação para a Ciência e a Tecnologia, IP, in the context of the program-contract described in paragraphs 4, 5, and 6 of art. 23 of Law no. 57/2016, of August 29th, as amended by Law no. 57/2017 of July 19th
Re-Design de tecidos estreitos para aplicações inovadoras
O conceito de qualidade de vida surge pela primeira vez em 1920, através do economista
inglês Arthur Cecil Pigou, que utiliza este termo para descrever o impacto governamental
sobre a vida das pessoas mais desfavorecidas.
Com a instalação de uma era industrializada e com o fim da 2º Guerra Mundial, a sociedade
mudou de paradigma e iniciou uma procura incessante de formas para melhorar a sua
qualidade de vida. Este conceito desenvolve-se juntamente com o desenvolvimento do
conceito de educação, saúde, habitação, transporte, trabalho e lazer, bem como indicadores
do aumento da esperança de vida, a diminuição da mortalidade infantil e dos nÃveis de
poluição.
O avanço da tecnologia teve um papel fundamental para a evolução desses conceitos, bem
como o Design na procura de soluções para aplicação dessas mesmas tecnologias. No caso
concreto da indústria tèxtil, a tendência é o desenvolvimento de têxteis inteligentes
envolvendo a engenharia electrónica no seu processo de conceptualização e de fabrico. A
chamada tecnologia wearable abre novos horizontes para a criação de soluções inovadoras,
abrindo novos nichos de mercado com elevado valor acrescentado.
Existem atualmente vários produtos no mercado cuja funcionalidade e utilidade lhes conferiu
um estatuto imutável ao longo dos anos, onde a evolução não avançou na tendência atual.
Esse é o caso dos tecidos estreitos, cuja funcionalidade poderá adquirir novas capacidades e
ser utilizada em diferentes componentes têxteis nas mais variadas áreas. Essas capacidades
poderão ser acrescentadas pela incorporação de materiais com luminosidade (Led’s e L-Wire)
nas suas estruturas. Neste estudo realizado o design de produtos com novas funcionalidades, adaptando as tecnologias até
agora desenvolvidas em novas soluções e/ou novas recriações de produto.The concept of quality of life appears in 1920, through the british economist Arthur Cecil Pigou, who uses this term to
describe the government's impact on the lives of the most underprivileged. With the installation of the industrialized era and
the end of the World War two, the society changed the paradigm and began a constant search for ways to improve their
quality of life. This concept is developed along with the development of the concept of education, health, housing,
transportation, work and leisure, as well as indicators of increased life expectancy and decreased infant mortality. The
evolution of technology had played a key role in the evolution of these concepts as well as the Design in finding solutions for
application of these technologies. In the case of the textile industry the trend is the development of smart textiles involving
electronic engineering in the process of conceptualizing and manufacturing. The result is the wearable technology that open
new horizons for creating new solutions, opening new market niches with high added value. Currently there are several
products on the market whose functionality and utility gave them an immutable status over the years, and the product
evolution has not advanced in the current trend. This is the case of ribbon, whose functionality can acquire new skills and be
used in different textile components in various areas. These capabilities may be added by the incorporation and variation of
color. As an example with the ability to become photochromatic materials (with a variation of light), thermochromic (with
temperature variation), electrochromic (with electricity variance) mecanic-chromic (with voltage variation and / or strain)
and also the possibility of incorporating luminescent coatings and the incorporation of luminescent materials in the
structure. In this study will be developed an product design with new features and adapt the technologies so far developed
in new solutions and / or new product recreationsEste trabalho foi desenvolvido no âmbito do projeto n.º 2014/37658 “NarrowTECH – Tecidos estreitos multifuncionais “, liderado pela empresa IDEPA e co-financiado pelo Fundo Europeu de Desenvolvimento Regional FEDER, através do COMPETE – Programa Operacional Fatores de Competitividad
Late Strunian age : a key time frame for VMS deposit exploration in the Iberian Pyrite Belt
Estimate of geological environments favorable for the formation of massive sulphide deposits is an important goal to the exploration companies working in the
Iberian Pyrite Belt (IPB), the main European VMS base metals province, with giant deposits such as Neves Corvo, Aljustrel (Portugal), Rio Tinto and Tharsis
(Spain). Palynostratigraphic research programs using more than 40 exploration boreholes (>30 km length) allowed the dating of the sediments of the
Volcano-Sedimentary Complex (upper Devonian to upper Viséan), that host the massive sulphide deposits. Research is based on detailed palynomorphs study.
Careful work was focused on dark grey and black shale units that host stockwork and massive ore mineralizations. Felsic volcanic U/Pb age data was also used
to confirm the sediment age.Late Strunian (miospore biozone LN, 360.7 ± 0.7 Ma -362 Ma) sediments host the massive sulphide ore at Neves Corvo, Lousal,
Caveira and Montinho, mines located in the Portuguese IPB sector. In Spain similar data was obtained at Aznalcollar and Tharsis. The agedata show a
favourable geological period of ~2 Ma were paleogeographic conditions were extremely favorable to hydrothermal fluid circulation and VMS deposits formation.
Late Strunian age therefore becomes one key exploration guide in IPB
Potential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrients
High-fat diet-induced obesity detrimentally affects brain function by inducing chronic low-grade inflammation. This neuroinflammation is, at least in part, likely to be mediated by microglia, which are the main immune cell population in the brain. Microglia express a wide range of lipid-sensitive receptors and their activity can be modulated by fatty acids that cross the blood-brain barrier. Here, by combining live cell imaging and FRET technology we assessed how different fatty acids modulate microglia activity. We demonstrate that the combined action of fructose and palmitic acid induce Ikβα degradation and nuclear translocation of the p65 subunit nuclear factor kB (NF-κB) in HCM3 human microglia. Such obesogenic nutrients also lead to reactive oxygen species production and LynSrc activation (critical regulators of microglia inflammation). Importantly, short-time exposure to omega-3 (EPA and DHA), CLA and CLNA are sufficient to abolish NF-κB pathway activation, suggesting a potential neuroprotective role. Omega-3 and CLA also show an antioxidant potential by inhibiting reactive oxygen species production, and the activation of LynSrc in microglia. Furthermore, using chemical agonists (TUG-891) and antagonists (AH7614) of GPR120/FFA4, we demonstrated that omega-3, CLA and CLNA inhibition of the NF-κB pathway is mediated by this receptor, while omega-3 and CLA antioxidant potential occurs through different signaling mechanisms.info:eu-repo/semantics/publishedVersio
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